Prosedur Double Stain

Prosedur Double Stain

No. Tahapan Waktu
1  Potong jaringan 4mm
2 Keringkan 370C. Panaskan diatas slide warmer 600C 30 menit
3 Deparafinasi Xylene I, xylene II, xylene III  @ 3 menit
4 Rehidrasi Alkohol abs, 96%, 80%, 70% @ 5 menit
5 Cuci air mengalir 5 menit
6 Blocking Endogen Peroksidase  5 – 10 menit
7 Cuci air mengalir 5 menit
8 Antigen Retrieval (NXGen Decloaker) Lymphoma-(solid tumor non fatty or
breast tissue) 110°/15-30 minutes
All other tissues that are not Fatty, skin or
breast 110°/15-30 minutes
Breast tissue 95°/40 minutes
Brain- 80°/60 minutes
9 Cuci TBS 5 menit
10 Blocking Sniper block (casein) 5 – 10 menit
11 Antibodi Primer/Cocktail Antibodi 60 menit
12 Cuci TBS 5 menit
13 Polymer MACH2 Double Stain  30 menit
14  Cuci TBS  5 menit
15 Chromogen DAB  5 menit
16 Cuci TBS 5 menit
17 Chromogen Vulcan Fast Red (mix before use) 15 menit
18 Counterstain Hematoxylin  5 menit
19 Cuci Air
20 Cuci TBS
21 Celupkan ke Xylene
22 Mounting

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